📊 24 U.S. AI Startups Have Already Raised $100M+ in 2025

Despite recurring questions about AI saturation, U.S. venture funding remains robust. With 24 mega-rounds by mid‑June, investors continue to pour capital into companies building infrastructure across enterprise search, generative models, healthcare AI, and more. The ongoing presence of billion-dollar rounds suggests capital is flowing toward companies with differentiated tech, strong metrics, and credible paths to commercialization.

Key Takeaways

1. Momentum is real: Last year saw 49 such rounds; mid‑year 2025 is already halfway there, signaling continued demand for generative AI plays.

2. Billion-dollar surge: Q1 2025 saw more mega-rounds than the same period in 2024, confirming investor appetite isn’t cooling.

3. Gold-standard companies: Glean’s $7.25B valuation spotlights enterprise AI’s pull, with search and NLP applications remaining investor favorites.

4. Broader AI ecosystem: Deals span diverse sub-sectors—healthcare (e.g., Hippocratic AI), creative tools (e.g., Runway), and enterprise productivity—underscoring AI’s breadth.

5. Strategic signaling: These rounds are as much signaling as financial—backing teams positioned to lead in infrastructure, compliance, and developer enablement.

Final Thoughts

The steady stream of nine- and ten-figure rounds underlines a maturing yet expanding AI investment environment. As we enter Q3, the key signals to track will be which of these high‑capital startups begin to monetize at scale—and whether the flow of mega-rounds accelerates or consolidates. The rest of 2025 will reveal whether the flood of capital begins to translate into tangible returns—or if investors begin to exercise more selectivity.